Coding

Part:BBa_K1675003:Design

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)


glutamate decarboxylase A （GadA）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of TTT to TTC Phe.(site:129bp)


Source

Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3666180&to=3667580&strand=true

References

[1]Shin S, Castanie‐Cornet M, Foster, John W, et al. An activator of glutamate decarboxylase genes regulates the expression of enteropathogenic Escherichia coli virulence genes through control of the plasmid-encoded regulator, Per.[J]. Molecular Microbiology, 2001, 41(5):1133-1150(18).

[2]Castanie-Cornet M P, Foster J W. Escherichia coli acid resistance: cAMP receptor protein and a 20 bp cis-acting sequence control pH and stationary phase expression of the gadA and gadBC glutamate decarboxylase genes.[J]. Microbiology, 2001, 147(Pt3):709-715.